ExoQuick Ultra (20 rxns)
Ultra-High EV Recovery
- Delivers up to 420× more EVs compared to ultracentrifugation.
- Isolates significantly more EVs per normalized input volume than competitor methods.
Ultra-Clean Preparations
- Reduces albumin carryover by up to 75%.
- Reduces immunoglobulin contamination by up to 40%.
- Cleaner EV preparations than ultracentrifugation and competing kits.
Superior Biomarker Detection
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Increases EV biomarker detection sensitivity by :
- Up to 11-fold for CD9
- Up to 8-fold for CD8
- Enables detection of low-abundance EV markers that may be missed with standard methods.
ExoQuick ULTRA vs. Top Competitor vs. Ultracentrifugation
Figure 1. ExoQuick® ULTRA yields highly pure, high-abundance exosomes.
(A) Coomassie blue-stained SDS-PAGE analysis comparing exosome preparations obtained using different isolation methods demonstrates that ExoQuick® ULTRA produces a cleaner protein profile, characterized by fewer and more discrete bands, relative to alternative methods.
(B) Western blot analysis of the same samples reveals that ExoQuick® ULTRA–isolated exosomes contain the highest levels of established exosomal markers CD9, CD81, and Hsp70, while showing minimal contamination from carryover proteins such as albumin and IgGH. In contrast, samples prepared using a competitor method (Company Q) are dominated by albumin, and ultracentrifugation-derived samples exhibit substantially higher levels of both albumin and IgGH.
All lanes were normalized and loaded with 7 µg of total protein, as quantified using a fluorometric Qubit protein assay.
Figure 2. Fluorescent nanoparticle tracking analysis (fNTA) confirms the superior EV yields obtained with ExoQuick® ULTRA compared to ultracentrifugation.
Extracellular vesicle (EV) recovery achieved using different isolation methods was quantitatively compared by (A) normalized input serum volume (particles per mL of serum) and (B) normalized input serum protein content (particles per mg of protein, as determined by a fluorometric Qubit protein assay). EV particle concentration was measured using fluorescent nanoparticle tracking analysis (fNTA), a highly specific technique for EV detection that minimizes interference from non-vesicular particles. These results demonstrate that ExoQuick® ULTRA delivers significantly higher EV yields than ultracentrifugation across both normalization strategies.
4 Simple Stages - 20 Minutes Hands-On-Time
Uncompromising EV isolation delivers higher yields and cleaner preps
Transmission electron microscopy (TEM) images of EVs isolated from human serum using ExoQuick® ULTRA are shown at two different magnifications. In both views, multiple vesicles displaying the size, shape, and membrane structure typical of extracellular vesicles are clearly observed, confirming the integrity and morphological consistency of the isolated EVs.