ExoQuick Ultra TC (20 rxns)
ExoQuick-TC® ULTRA is the most advanced and efficient method for isolating extracellular vesicles (EVs) from a wide range of biofluids. Combining exceptional purity, high recovery, and a streamlined workflow, this next-generation system outperforms ultracentrifugation and competitor kits in yield, cleanliness, and biomarker sensitivity.
Ultra-Clean EV Preparations
- Reduces albumin carryover by up to 75%
- Reduces immunoglobulin contamination by up to 40%
- Cleaner EV isolates than ultracentrifugation and competing kits
Higher EV Yields
- Recovers more EVs per normalized input volume
- Consistently outperforms ultracentrifugation and alternative methods
Enhanced Biomarker Detection
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Improves EV marker sensitivity by:
- Up to 11-fold for CD9
- Up to 8-fold for CD81
- Enables detection of low-abundance EV biomarkers
Uncompromising EV isolation delivers higher yields and cleaner preps
How It Works ?
How to get to ULTRA-clean EV preps ?
The ExoQuick-TC ULTRA protocol is quick and easy, requiring only 10-minutes of hands-on time.
- Add ExoQuick-TC ULTRA to 5 mL of tissue culture media or other biofluid and incubate overnight at 4°C
- Collect EVs by spinning at 3,000g for 10 minutes
- Resuspend EVs and add to pre-washed ExoQuick-TC ULTRA columns
- Spin 1,000g x 30 sec and collect EVs, they are now ready to use
See how well ExoQuick ULTRA delivers ?
Figure 1. ExoQuick-TC® ULTRA produces high-yield, high-purity exosome preparations.
Western blot analysis comparing extracellular vesicle (EV) isolation methods demonstrates that ExoQuick-TC® ULTRA yields the highest levels of the exosome-specific marker CD63 while exhibiting minimal contamination from the carryover protein albumin. In contrast, samples prepared using a competitor method (Company Q) are predominantly albumin, and even ultracentrifugation-derived preparations contain substantially higher levels of both albumin and IgGH.
All lanes were normalized and loaded with 4 µg of total protein.
Figure 2. Fluorescent nanoparticle tracking analysis (fNTA) confirms the superior EV yields obtained with ExoQuick® ULTRA compared to ultracentrifugation.
Extracellular vesicle (EV) recovery from serum was quantitatively compared across different isolation methods, normalized by (A) input serum volume (particles per mL) and (B) input serum protein content (particles per mg of protein, as measured using a fluorometric Qubit protein assay). EV particle concentration was determined using fluorescent nanoparticle tracking analysis (fNTA), a highly specific method that selectively detects EVs while minimizing interference from non-vesicular particles. These results demonstrate that ExoQuick® ULTRA consistently delivers higher EV yields than ultracentrifugation across both normalization strategies.
Figure 3. Extracellular vesicles isolated using ExoQuick® ULTRA exhibit characteristic EV morphology.
Transmission electron microscopy (TEM) images of extracellular vesicles (EVs) isolated from human serum using ExoQuick® ULTRA are shown at two different magnifications. In both views, multiple vesicles displaying the size, shape, and membrane features typical of EVs are clearly observed, confirming the structural integrity of the isolated vesicles.