ExoBacteria OMV Kit (20 rxns)
This dedicated OMV isolation system enables researchers to obtain clean, high-yield preparations of bacterial outer membrane vesicles (OMVs) using a streamlined, user-friendly workflow. Designed specifically for bacterial samples, it delivers superior performance compared to traditional ultracentrifugation-based methods.
The only kit for easy isolation of bacterial outer membrane vesicles (OMVs)
Transmission electron micrograph (TEM) of OMVs isolated using the ExoBacteria OMV Isolation Kit (light blue arrows).
See why the ExoBacteria OMV Isolation Kit is a better way to isolate OMVs
Figure 1. ExoBacteria OMV Isolation Kit produces uniform OMVs with reduced protein contamination compared to ultracentrifugation.
Transmission electron microscopy (TEM) images compare E. coli–derived outer membrane vesicles (OMVs) isolated using (A) the ExoBacteria OMV Isolation Kit and (B) traditional ultracentrifugation. OMVs (indicated by light blue arrows) are visible in both preparations; however, OMVs isolated with the ExoBacteria kit display a tighter size distribution and cleaner background. In contrast, ultracentrifugation-derived samples show the presence of undesirable protein aggregates (indicated by red arrows), highlighting increased contamination relative to the kit-based method.
Figure 2. ExoBacteria OMV Isolation Kit yields OMVs with a narrower size distribution and higher recovery than ultracentrifugation.
(A) Fluorescent nanoparticle tracking analysis (fNTA) of E. coli–derived outer membrane vesicles (OMVs) isolated using the ExoBacteria OMV Isolation Kit (blue trace) or ultracentrifugation (gray trace) demonstrates that the kit produces OMVs with a tighter and more uniform size distribution compared to the conventional ultracentrifugation method.
(B) Quantitative analysis further shows that the total OMV yield obtained with the ExoBacteria OMV Isolation Kit is approximately 20-fold higher (1.38 × 10⁸ particles/µg protein) than that achieved by ultracentrifugation (0.0657 × 10⁸ particles/µg protein).
Figure 3. ExoBacteria OMV Isolation Kit provides higher OMV yields with reduced protein contamination compared to ultracentrifugation.
Western blot analysis of E. coli–derived outer membrane vesicles (OMVs) isolated using either the ExoBacteria OMV Isolation Kit or ultracentrifugation demonstrates that (A) the kit yields a greater amount of OMVs, as indicated by increased levels of the OMV-associated marker GroEL, and (B) kit-prepared samples contain lower levels of carryover protein, as evidenced by reduced flagellin signal relative to ultracentrifugation-derived preparations. Each lane was loaded with 5 µg of total protein.
Figure 4. ExoBacteria OMV Isolation Kit enables broader and more specific OMV proteome identification.
(A) Mass spectrometry–based proteomic analysis comparing outer membrane vesicles (OMVs) isolated using the ExoBacteria OMV Isolation Kit or ultracentrifugation demonstrates that the kit-based method yields a greater number and broader diversity of identified proteins. Notably, all but seven proteins detected in ultracentrifugation-isolated OMVs were also identified in OMVs isolated using the ExoBacteria kit, indicating that the higher yield and improved purity achieved with the kit enhance proteomic coverage and analytical depth.
(B) A higher proportion of proteins identified from ExoBacteria OMV Isolation Kit–derived OMVs matched entries in the EV-Proteome / EV-Pedia database, further demonstrating the increased specificity and biological relevance of OMVs isolated using the ExoBacteria OMV Isolation Kit compared to ultracentrifugation.