SmartSEC HT
SmartSEC™ HT is the first 96-well plate–based extracellular vesicle (EV) isolation system, enabling high-purity, high-yield EV isolation from serum and plasma at true high throughput. Designed to accelerate EV biomarker discovery, diagnostics, and therapeutic research, SmartSEC™ HT combines the performance of size-exclusion chromatography with the scalability required for large sample cohorts.
How It Works ?
Isolate EVs from up to 96 samples in under an hour
See the purity and yields achievable with SmartSEC HT
Figure 1. SmartSEC™ HT yields high-purity extracellular vesicles with minimal protein carryover.
Extracellular vesicles (EVs) were isolated from 500 µL of serum (A) or 500 µL of plasma (B) using the indicated isolation methods. For Western blot analysis, 1 µg protein equivalent from the first EV-containing fraction was loaded per lane. SmartSEC™ HT produced EV preparations with higher yields and reduced carryover protein compared to ultracentrifugation and a competitor “q” size exclusion chromatography (SEC) column.
Figure 2. Most extracellular vesicles elute in the first two SmartSEC™ HT fractions.
As is characteristic of size exclusion chromatography (SEC), EV elution profiles depend on both input sample volume and eluent volume. When 250 µL of plasma was processed (A), fraction 2 contained the highest EV concentration, whereas with 500 µL of plasma (B), fraction 1 contained the highest EV concentration. For initial use of SmartSEC™ HT, it is recommended to collect fractions 1 and 2 separately, analyze EV content, and determine whether pooling is appropriate based on experimental requirements.
Figure 3. SmartSEC™ HT delivers superior EV yields compared to ultracentrifugation and a competitor SEC method.
Extracellular vesicles (EVs) were isolated from 250 µL and 500 µL of serum and plasma using the indicated methods. EV yield was quantified using (A) a Qubit protein assay and (B) fluorescent nanoparticle tracking analysis (fNTA). Table 1 summarizes the corresponding quantitative values. Across both input volumes and analytical methods, SmartSEC™ HT consistently outperformed ultracentrifugation and a competitor “q” size exclusion chromatography (SEC) column in EV recovery.